Stefano Schiaffino - CV

Contact information
email: stefano.schiaffino@unipd.it

Department of Biomedical Sciences, Viale G. Colombo 3, 35121 Padova, Italy
tel. +39.049.827-6034; fax +39.49.827-6040

Venetian Institute of Molecular Medicine (VIMM), Via Orus 2, 35129 Padova, Italy
tel. +39.049.7923232; fax +39.049.7923232

Education
1963 M.D., University of Modena

Appointments/Affiliations
2002-Present Head, Laboratory of Neuromuscular Biology and Physiopathology of the Consiglio Nazionale delle Ricerche (CNR) Institute of Neuroscience
2000-Present Vice President, Venetian Institute of Molecular Medicine (VIMM)
1981-Present Professor of General Pathology, School of Medicine, University of Padova
1988-2002 Director, Consiglio Nazionale delle Ricerche (CNR) Center of Muscle Biology and Physiopathology
1986-87 Visiting scientist, INSERM U 127, Hopital Lariboisière, Paris
1971-81 Associate Professor of General Pathology, School of Medicine, Univ. Padova
1965-71 Assistant Professor of General Pathology, School of Medicine, Univ. Padova

Honors and Awards
1997 Doctor honoris causa in Medicine, University of Paris VII
1996 Accademia Nazionale dei Lincei (Roma)
1992 Istituto Veneto di Scienze, Lettere ed Arti (Venezia)
1989 Accademia Galileiana di Scienze, Lettere ed Arti (Padova)
1988 Doctor honoris causa in Medicine, University of Umeå (Sweden)

Membership
1999- Editorial Board, Journal of Muscle Research and Cell Motility
1999-2003 Vice-President, Scientific Committee of TELETHON Italy
1998-2002 Scientific Council, Association Francaise contre les Myopathies (AFM)
1994-2000 Editorial Board, Circulation Research
1995-99 Scientific Council, Institut Fédératif de Recherche CNRS- INSERM "Circulation-Lariboisière", Parigi

Research field
Muscle gene regulation - Neuromuscular signalling in vivo

Publications (selected, 1993-2004)

McCullagh K, Calabria E, Pallafacchina G, Ciciliot S, Serrano AL, Argentini C, Kalhovde JM, Lømo T, Schiaffino S. (2004) NFAT is a nerve activity sensor and controls activity-dependent myosin switching. Proc. Nat. Acad. Sci. USA, 101:10590-95.
Sandri M, Sandri C, Gilbert A, Skurk C, Calabria E, Picard A, Walsh K, Schiaffino S, Lecker SH, Goldberg AL. (2004) Foxo transcription factors induce the atrophy-related ubiquitin ligase atrogin-1 and cause skeletal muscle atrophy. Cell 117:399-412.
Sandri C , Di Lisi R, Picard A, Argentini C, Calabria E, Myklak K, Scartezzini P, Schiaffino S (2004) Heart morphogenesis is not affected by overexpression of the Sh3bgr gene mapping to the Down syndrome heart critical region. Human Genet 114:517-519.
Moreno H, Serrano A, Santalucía T , Gumà A, Cantó C, Palacín M, Schiaffino S, Zorzano A. (2003) Differential regulation of the muscle-specific GLUT4 enhancer in regenerating and adult skeletal muscle. J Biol Chem 278:40557-64.
Salamon M, Millino C, Raffaello A, Mongillo M, Sandri C, Bean C, Negrisolo E, Pallavicini A, Valle G, Zaccolo M, Schiaffino S, Lanfranchi G. (2003) Human MYO18B, a novel unconventional myosin heavy chain expressed in striated muscles moves into the myonuclei upon differentiation. J. Mol Biol 326:137-49.
Schiaffino S, Serrano AL (2002) Calcineurin signaling and neural control of skeletal muscle fiber type and size. Trends Pharmacol Sci. 23: 569-575.
Pallafacchina G, Calabria E, Serrano AL, Kalhovde JM, Schiaffino S. (2002) A protein kinase B-dependent and rapamycin-sensitive pathway controls skeletal muscle growth but not fiber type specification. Proc Nat Acad Sci USA 99:9213-18.
Serrano AL, Murgia M., Pallafacchina G, Calabria E, Coniglio P, Lømo T, Schiaffino S. (2001) Nerve activity-dependent specification of slow skeletal muscle fibers is controlled by calcineurin. Proc Nat Acad Sci USA 98:13108-13.
Murgia M, Serrano AL, Calabria E, Pallafacchina G, Lømo T, Schiaffino S. (2000) Ras is involved in nerve-activity-dependent regulation of muscle genes. Nature Cell Biol. 2:142-147.
Egeo A, Di Lisi R, Sandri C, Mazzocco M, Lapide M, Schiaffino S, Scartezzini P. (2000) Developmental expression of the SH3BGR gene mapping to the Down syndrome heart critical region. Mech Dev 90:313-316.
Weiss A, Schiaffino S, Leinwand L (1999) Comparative sequence analysis of the complete human sarcomeric myosin heavy chain family: implications on functional diversity. J. Mol Biol 290: 61-75.
Schiaffino S, Dallapiccola B, Di Lisi R. (1999) Molecular genetics of congenital heart disease: a problem of faulty septation. Circ Res 84:247-249.
Di Lisi R, Millino C, Calabria E, Altruda F, Schiaffino S, Ausoni S. (1998) Combinatorial cis-acting elements control tissue-specific activation of the cardiac troponin I gene in vitro and in vivo. J Biol Chem 273, 25371-25380.
Gautel M, Furst D, Cocco A, Schiaffino S. (1998) Isoform transitions of the myosin-binding protein C family in developing human and mouse muscles. Lack of isoform transcomplementation in cardiac muscle. Circ Res 82: 124-129.
Schiaffino S, Salviati G (1997) Molecular diversity of myofibrillar proteins: isoforms analysis at the protein and mRNA level. Meth Cell Biol. 52: 349-369.
Schiaffino S. (1997) Protean patterns of gene expression in the heart conduction system. Circ Res 80: 749-750.
Schiaffino S, Reggiani C. (1996) Molecular diversity of myofibrillar proteins: gene regulation and functional significance. Physiol Rev 76: 371-423.
Guénet JL, Simon-Chazottes D, Gravel M, K. Hastings KEM, Schiaffino S. (1995) Cardiac and skeletal muscle troponin I isoforms are encoded by a dispersed gene family on mouse chromosomes 1 and 7. Mamm Genome 7, 13-15
Smerdu V, Karsch-Mizrachi I, Campione M, Leinwand L, Schiaffino S. (1994) Type 2X myosin heavy chain transcripts are expressed in type 2B fibers of human skeletal muscle. Am J Physiol. 267, 1723-1728.
Bottinelli R, Betto R, Schiaffino S, Reggiani C. (1994) Unloaded shortening velocity and myosin heavy chain and alkali light chain isoform composition in rat skeletal muscle fibres. J Physiol 478, 341-349.
Vitadello M, Schiaffino MV, Picard A, Scarpa M, Schiaffino S. (1994) Gene transfer in regenerating muscle. Hum Gene Ther 5, 11-18.
Ausoni S, Campione M, Picard A, Moretti P, Vitadello M, De Nardi C, Schiaffino S. (1994) Structure and regulation of the mouse cardiac troponin I gene. J Biol Chem 269, 339-346.
Schiaffino S, Reggiani C. (1994) Myosin heavy chain isoforms in mammalian skeletal muscle. J Appl Physiol 77: 493-501.
Schiaffino S, Gorza L, Ausoni S. (1993) Troponin isoform switching in the developing heart and its functional consequences. Trends Cardiovasc Med 3: 12-17.
DeNardi C, Ausoni S, Moretti P, Gorza L, Velleca M, Buckingham M, Schiaffino S. (1993) Type 2X myosin heavy chain is coded by a muscle fiber type-specific and developmentally regulated gene. J Cell Biol 123, 823-835.
Gorza L, Ausoni S, Merciai N, Hastings KEM, Schiaffino, S. (1993) Regional differences in troponin I isoform switching during rat heart development. Dev Biol 156, 253-264.
Gorza L, Schiaffino S, Volpe P. (1993) The inositol 1,4,5-triphosphate receptor in heart: evidence for its concentration in Purkinje myocytes of the conduction tissue. J Cell Biol 121, 345-353.

Invited speaker (selected, 1993-2004)
- Conference "Molecular and Cellular Biology of Muscle Development: in vivo analysis", Titisee (Germany), 03. 1993.
- 1st Meeting European Working Group on Human Gene Transfer and Therapy, Stresa, 11. 1993.
- INSERM U 127, Hopital Lariboisière, Parigi, 02. 1993.
- Centro di Biotecnologie Avanzate, Genova, 11. 1993.
- Keystone Symposium "Molecular Biology of Muscle Development", Snowbird (Utah), USA, 04. 1994.
- XV European Section Meeting, International Society for Heart Research, Copenhagen, 06. 1994.
- NATO International Workshop "Cardiac Growth and Regeneration", Viterbo, 06. 1994.
- Meeting "Molecular Biology Approaches for Gene Therapy in the Myocardium", La Bretesche, Francia, 07. 1994.
- International Workshop "Sarcomeric myosins: structure, function and disfunction", Venezia, 09. 1994 (organizer & speaker).
- '95 Meeting Norvegian Physiological Society, Rondablik, Norway, 02. 1995.
- XV World Congress Internat. Society for Heart Research, Praga, 07. 1995.
- XXIV European Muscle Conference, Firenze, 09. 1995.
- Symposium "Molecular Mechanisms Regulating Skeletal Muscle Plasticity", Airlie, Virginia, USA, 09. 1995.
- 4th International Symposium "Amino acid/Protein Metabolism in Health and Disease", (opening lecture), Padova, 04. 1996.
- Course "Control mechanisms for human movement and muscle energetics", Copenhagen, 06. 1996
- Symposium "Tissue Regeneration", 24th Meeting Federation of European Biochemical Societies (FEBS), Barcelona, 7-12. 07. 1996.
- 6th Colloque sur les Maladies Neuromusculaires, Versailles, 21-25.10. 1996.
- International Symposium "Molecular mechanisms of myofibril assembly", Chiba (Japan), 7-9.11. 1996
- Keystone Symposium "Molecular Biology of Muscle Development", Snowmass, Colorado, 1-6.04. 1996.
- 1st Annual Symposium Armenise-Harvard Foundation,18-20. 06. 1997.
- Symposium "Physiological Adaptation to Use" (organizer & speaker), XXXIII International Congress of Physiological Sciences, St. Petersburg, 30.06- 6.07.1997.
- Symposium "Muscle Plasticity", Scandinavian Physiological Society, Oslo, 13-16.11. 1997.
- European Science Foundation Workshop "Cardiovascular specific gene expression", Maastricht,12-14. 12. 1997.
- World Congress "International Society Heart Research", Rhode, 27-31. 05. 1998
- "Third Annual Congress of the European College of Sport Science", Manchester 15-18. 07. 1998.
- XX Congress of the European Society of Cardiology, Vienna, 22-26. 08. 1998.
- XXVII European Muscle Conference, Lund (Sweden) 11-16. 09. 1998.
- XXth meeting International Society for Heart Research, European section, Maastricht, 23-26. 06.1999.
- XV Congress Internat. Federation of Associations of Anatomists, Roma 11-16. 09. 1999.
- EMBO Workshop "Molecular Genetics of Muscle Development and Neuromuscular Diseases", Kloster Irsee, Germany, 26.09-1.10. 1999.
- International Conference "Myologie", Nice, 27-31. 03. 2000.
- Conference "The Molecular Biology of Muscle Development", Asilomar, CA, 21-26. 05. 2000.
- Armenise-Harvard Foundation, 4th Annual Symposium, Bretton Woods, 19-20.06. 2000.
- EMBO Workshop on "Myogenesis", Il Ciocco (Lucca) 29.04-4.05. 2001.
- Gordon Research Conference "Biology of Aging", Oxford) 22-27. 07. 2001.
- XXX European Muscle Conference, Pavia, 8-13. 09. 2001
- Xth International Congress on Neuromuscular Diseases, Vancouver, 7-12. 07. 2002
- EURESCO Conference "Cellular and Molecular Basis of Regeneration", Il Ciocco (Lucca) 30.08- 5.09. 2002.
- EMBO Workshop "Molecular Genetics of Myogenesis and Muscular Diseases", Cambridge 29.09-3.10. 2002.
- TELETHON Convention, Riva del Garda, 24-26. 11. 2002
- Federation European Physiological Societies (FEPS), Nice 28.06- 3.07. 2003.
- 8th CONGRESS World Muscle Society, Szeged (Hungary) 3-6. 09. 2003
- Junior Faculty Lecture, Nobel Forum, Karolinska Institute, Stockholm, 19. 09. 2003
- IOC Workshop on "Genes, performance and health", Athens, 7. 10. 2003
- Lettura del Presidente, Congress FISV/ABCD, Rimini, 11. 10. 2003
- Juan March Workshop "The Calcium/Calcineurin/NFAT Pathway: Regulation and Function", Madrid 3-5. 11. 2003.
- Workshop Europ. Centre of Neuromuscular Diseases, Naarden (Holland), 28-30. 11. 2003
- Gordon Research Conference "Myogenesis", Il Ciocco (Lucca), 16-21. 05. 2004.
- 13th European Congress on Obesity, Praga, 26-29. 05. 2004.
- 33rd European Muscle Conference, Symposium "Intracellular transduction signal", Isola d'Elba, 19-23. 09. 2004 (Chairman & speaker).
- Killam Seminar, Montreal Neurological Institute, Mc Gill University, Montreal, 23. 11. 2004.